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Assembly of active Cdc28-cyclin complexes in vitro is independent of Cdc37. The indicated combinations of purified Cdc28-HA, GST-Clb2, human CAK, and Cdc37 were incubated for 15 min on ice and then for 15 min at 23°C. The resulting histone H1 kinase activity was measured in solution. In the final lane, Cdc37 was not included in the incubation step but added immediately prior to the histone H1 kinase assay.
