FIG. 2.
(A) Northern analysis of mRNA from β5+/+ and β5−/− mice. Total RNA was extracted from cultured keratinocytes and transcribed to cDNA. The specific cDNA probe of mouse β5 was used to detect message from wild-type but not deficient cells. (B) Immunoprecipitation of αvβ5 from wild-type but not β5−/− murine keratinocytes. [35S]methionine-labeled keratinocyte lysate was immunoprecipitated with anti-β5 antibody raised against the human β5 cytoplasmic domain. Immunoprecipitated proteins were analyzed by SDS-PAGE under nonreducing conditions. The positions of molecular mass marker (in kilodaltons) are shown to the left.