FIG 5.

Irgm2^−/− and pan-Irgm^−/− mice are defective for immunity to Toxoplasma in vitro and in vivo. Wild-type, Irgm2^−/−, and pan-Irgm^−/− MEFs were primed with IFN-γ overnight and infected with GFP-Toxoplasma for 72 h prior to quantification of plaque formation. Graphs represent plaque numbers in IFN-γ-primed cells relative to those in unprimed cells of the same genotype (mean ± SEM for at least three independent experiments). Statistical significance was determined using one-way ANOVA with Tukey’s multiple-comparison test, comparing normalized plaque formation percentages of IFN-γ-treated samples. (A). Wild-type, Irgm2^−/−, and pan-Irgm^−/− mice were infected intraperitoneally with Toxoplasma, and survival was monitored over 21 days. Wild-type n = 17; Irgm2^−/− n = 20; pan-Irgm^−/− n = 15. Graph represents combined data from two independent experiments. Statistical significance was determined using the log-rank test (B). *, P < 0.05; **, P < 0.005; ***, P < 0.0005; ****, P < 0.00005; n.s., not significant.