TABLE 1.

Select differentially expressed genes in ΔliaF and ΔliaR isogenic mutants relative to that in the parental strain, MGAS10870

Locusa	Geneb	Predicted product	Fold changec
			ΔliaF	ΔliaR
	speC	Streptococcal pyrogenic exotoxin, C	−3.35	2.08
SpyM3_0098	ap-1	Pilus, accessory protein-1	−2.03	4.40
SpyM3_0099	srtA1	Pilus, sortase A1	−1.87	4.06
SpyM3_0100	tee3	Pilus, backbone protein (tee)	−1.93	4.07
SpyM3_0101	srtA2	Pilus, sortase A2	−1.89	3.26
SpyM3_0102	ap-2	Pilus, accessory protein-2	−1.62	3.05
SpyM3_0103	msmR	Multiple sugar metabolism regulator	−1.78
SpyM3_0298	prtS	IL-8 protease (PrtS/SpyCEP)	3.09	−17.98
SpyM3_0583	mac-1	IgG protease (Mac-1/IdeS)	2.97	−10.50
SpyM3_1032	grab	Protein G-related α2-macroglobulin-binding protein	−1.87	2.07
SpyM3_1301	speA3	Streptococcal pyrogenic exotoxin, A3	2.70	−3.96
SpyM3_1409	sdn	Streptodornase (Sdn)	−3.64
SpyM3_1698	ska	Streptokinase A precursor	2.13	−7.54
SpyM3_1728	mga	M protein trans-acting positive regulator	1.62	−2.16
SpyM3_1742	speB	Secreted cysteine protease		432.03
SpyM3_1744	ropB	Regulator of proteinase B	−1.76	2.38
SpyM3_1799	spxA2	Transcriptional regulator Spx	4.89	−12.07
SpyM3_1851	hasA	Hyaluronan synthase (capsule)	1.73	−15.54
SpyM3_1852	hasB	UDP-glucose 6-dehydrogenase (capsule)	1.68	−15.81
SpyM3_1853	hasC	UDP-glucose pyrophosphorylase (capsule)	1.68	−14.10

^aLocus tag designation in the reference genome MGAS315.

^bGene names in bold font indicate predicted or known regulation by CovRS.

^cValues for ΔliaF and ΔliaR are fold-change relative to the parental strain, MGAS10870. Only fold-change values with a P value <0.05 (Bonferroni corrected for multiple comparisons) are shown in the table.