FIG 8.

Bacterial replication as a function of time and Zn availability. The 129S6 BMDMs were infected with Salmonella Typhimurium expressing pDiGc. The GFP and DsRed signals in BMDMs were measured by flow cytometry and plotted against one another. These density graphs show only macrophages containing GFP⁺ Salmonella Typhimurium, which indicates active infection. Percentages on each plot indicate the number of macrophages with diluted DSRed signal, an indicator of Salmonella Typhimurium division. Dilution of DsRed is a function of time and zinc availability, with all media conditions showing an increase in bacterial division over time, but control and zinc-replete conditions (first and second rows) show much more robust division. Figure created with BioRender.com.