TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant characteristics | Source |
|---|---|---|
| Strains | ||
| E. coli | ||
| Top10 | F– mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG | Invitrogen |
| χ6212 | F– λ– ϕ80 Δ(lacZYA-argF) endA1 recA1 hsdR17 deoR thi-1 glnV44 gyrA96 relA1 ΔasdA4 | 85 |
| SM10(λpir) | Kmr; thi-1 thr-1 leuB26 tonA21 lacY1 supE44 recA integrated RP4-2 Tcr::Mu aphA+ (RP4-2 is RP4 ΔTn1) | 86 |
| RHO3 | Kms; SM10(λpir) Δasd::FRT ΔaphA::FRT | 87 |
| P. aeruginosa | ||
| PA103 | Wild-type strain | Joanna B. Goldberg |
| PAO1 | Wild-type strain | Shouguang Jin |
| AMC-PA10 | Clinical isolate from a patient sputum sample; resistant to piperacillin-tazobactam, ceftazidime, cefepime, aztreonam, ciprofloxacin, amikacin | Albany Medical Center |
| PA103 ΔexoU | ΔexoU | 88 |
| PA-m1 | ΔlpxL1 | This study |
| PA-m2 | ΔexoU ΔexoA | This study |
| PA-m3 | ΔexoU ΔexoA ΔexoT | This study |
| PA-m4 | ΔexoU ΔexoA ΔexoT ΔlasA | This study |
| PA-m5 | ΔexoU ΔexoA ΔexoT ΔlasA ΔlasB | This study |
| PA-m6 | ΔexoU ΔexoA ΔexoT ΔlasA ΔlasB ΔwbjA | This study |
| PA-m7 | ΔexoU ΔexoA ΔexoT ΔlasA ΔlasB ΔwbjA ΔpchA | This study |
| PA-m8 | ΔexoU ΔexoA ΔexoT ΔlasA ΔlasB ΔwbjA ΔpchA ΔphzM | This study |
| PA-m9 | ΔexoU ΔexoA ΔexoT ΔlasA ΔlasB ΔwbjA ΔpchA ΔphzM Δalg | This study |
| PA-m10 | ΔexoU ΔexoA ΔexoT ΔlasA ΔlasB ΔwbjA ΔpchA ΔphzM Δalg ΔrhlAB | This study |
| PA-m11 | ΔexoU ΔexoA ΔexoT ΔlasA ΔlasB ΔwbjA ΔpchA ΔphzM Δalg ΔrhlAB ΔpvdA | This study |
| PA-m12 | ΔexoU ΔexoA ΔexoT ΔlasA ΔlasB ΔwbjA ΔpchA ΔphzM Δalg ΔrhlAB ΔpvdA ΔplcH | This study |
| PA-m13 | ΔexoU ΔexoA ΔexoT ΔlasA ΔlasB ΔwbjA ΔpchA ΔphzM Δalg ΔrhlAB ΔpvdA ΔplcH ΔphoA | This study |
| PA-m14 | ΔexoU ΔexoA ΔexoT ΔlasA ΔlasB ΔwbjA ΔpchA ΔphzM Δalg ΔrhlAB ΔpvdA ΔplcH ΔphoA ΔlpxL | This study |
| Plasmids | ||
| pYA3342 | Asd+ vector, Ptrc, pBR ori | 85 |
| pYA3493 | Asd+ vector with β-lactamase N-terminal signal sequence, Ptrc, pBR ori | 85 |
| pDMS197 | Suicide vector; Tetr; mob (RP4) R6K ori, sacB | 82 |
| pUCP20 | E. coli-Pseudomonas shuttle vector; Apr Cbr | 89 |
| pSMV81 | The pcrV-hitAT DNA fragment was cloned into EcoRI and HindIII sites in pYA3494 | This study |
| pSMV82 | The pcrV-hitAT-6×His fragment was cloned into NcoI and HindIII sites in pYA3342 | This study |
| pSMV83 | The Ptrc-bla ss-pcrV-hitAT DNA fragment from pSMV81 was cloned into pUCP20 | This study |