FIG 2.

Dynamics of ILC numbers in FGT parts per mg tissue upon infection with C. muridarum. wt mice were infected intravaginally with 5 × 10⁵ IFUs of C. muridarum. FGTs were isolated at the indicated time points, and leucocytes of the various FGT parts were analyzed by immunostaining and flow cytometry. Absolute cell numbers were obtained by including a defined number of reference beads and are represented relative to the corresponding tissue weight. (A) cNK cells were defined as CD45⁺, Lin(CD3, CD5, CD19, TCRβ, TCRγδ, F4/80, FcεR1α, Ly6G)⁻, NK1.1⁺, Eomes⁺, CD49a⁻ cells. (B) trNK cells were defined as CD45⁺, Lin⁻, NK1.1⁺, Eomes⁺, CD49a⁺ cells. (C) ILC1s were defined as CD45⁺, Lin⁻, NK1.1⁺, Eomes⁻, CD49a⁺ cells. (D) ILC2s were defined as CD45⁺, Lin⁻, CD127⁺, ST2⁺, GATA3⁺ cells. Panels on the left give total cell numbers and panels on the give cell numbers per tissue weight. Data show means and SEM of 3 to 9 mice (only two mice for uninfected uterine horns, group 1 ILCs). The numbers of mice and experiments are separately listed in Table S1. Significance between means was tested by Tukey’s multiple-comparison test (*, P < 0.05; other differences were not significant). ILC, innate lymphoid cell; cNK, conventional natural killer; trNK, tissue-resident natural killer; dpi, days postinfection.