FIG 6.

Correlation of the absolute numbers of ILCs or CD45-negative cells and the corresponding oviduct weight. wt mice were infected intravaginally with 5 × 10⁵ IFUs of C. muridarum. FGTs were isolated at 30 dpi, and leucocytes of FGT oviducts were analyzed via immunostaining and subsequent flow cytometry. Data points represent the absolute number of cells in the oviduct, which were obtained by including a defined number of reference beads, and corresponding oviduct weights. (A) cNK cells were defined as CD45⁺, Lin(CD3, CD5, CD19, TCRβ, TCRγδ, F4/80, FcεR1α, Ly6G)⁻, NK1.1⁺, Eomes⁺, CD49a⁻ cells. (B) trNK cells were defined as CD45⁺, Lin⁻, NK1.1⁺, Eomes⁺, CD49a⁺ cells. (C) ILC1s were defined as CD45⁺, Lin⁻, NK1.1⁺, Eomes⁻, CD49a⁺ cells. (D) ILC2s were defined as CD45⁺, Lin⁻, CD127⁺, ST2⁺, GATA3⁺ cells. (E) Nonhematopoietic cells were defined as CD45 negative. The exact numbers of mice and experiments are separately listed in Table S1. Significance was tested by linear regression (*, P < 0.05; **, P < 0.01; ***, P < 0.001; n.s., P > 0.05). ILC, innate lymphoid cell; cNK, conventional natural killer; trNK, tissue-resident natural killer.