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. 2021 Oct 1;12:760041. doi: 10.3389/fpls.2021.760041

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Copyright © 2021 Zhu, Xing, Teng, Deng, Shen, Ai, Lu, Zhang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular localization and tissue expression patterns of OsRELA. (A) Subcellular localization of the OsRELA-GFP fusion protein in rice protoplasts. mCherry-NLS was used as the nuclear marker. Bar = 20 μm. (B) Fluorescent signals in transgenic rice plants expressing GFP-OsRELA. The nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Bar = 80 μm. (C) RT–qPCR analysis of the expression patterns of OsRELA in various tissues, including root, stem, leaf blade, lamina joint, leaf sheath and young panicle. The rice UBQ gene was amplified as the internal control. (D) Examination of GUS activity in transgenic plants expressing proOsRELA:GUS. GUS activity is found in the lamina joint, young florets, stem, vascular bundles, leaf sheath and root.