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. Author manuscript; available in PMC: 2021 Oct 15.
Published in final edited form as: Phytobiomes J. 2020 Oct 20;4(4):303–313. doi: 10.1094/pbiomes-02-20-0019-r

Fig. 1.

Fig. 1.

Schematic of hydroponic growth system for quantification and imaging of bacterial associations with the roots of Arabidopsis thaliana seedlings. (1) Two surface-sterilized seeds of A. thaliana were germinated on discs of sterile Teflon mesh for 7 to 10 days on top of agar. (2) Mesh discs embedded with two seedlings were transferred to liquid colonization medium, into which bacterial strains were inoculated. (3) After 20 h of colonization, the mesh and seedlings were rinsed and either (3a) the seedling was transferred to the liquid maintenance medium or (3b) the seedlings were immediately (4) removed from the multiwell plate for sample collection, to be either (5a) sonicated in liquid to resuspend bacteria for plating for CFU determinations or (5b) transferred to microscope slides for fluorescence microscopy imaging.