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. 2021 Oct 15;16(10):e0258427. doi: 10.1371/journal.pone.0258427

Table 1. Guide RNA sequences.

Name Target sequence Sequence for gRNA cloning Strand
DND1_gRNA-1 GCCCGTTCACCTGCACCAGG NGG CACCGCCCGTTCACCTGCACCAGG plus
AAACCCTGGTGCAGGTGAACGGGC
DND1_gRNA-2 GACAGGCATCCGCCTGGTGC NGG CACCGACAGGCATCCGCCTGGTGC minus
AAACGCACCAGGCGGATGCCTGTC
DND1_gRNA-3 GGCTGGGACTACCGTACCTG NGG CACCGGCTGGGACTACCGTACCTG plus
AAACCAGGTACGGTAGTCCCAGCC
DND1_gRNA-4 GCTTACCTCACAATCCCGCT NGG CACCGCTTACCTCACAATCCCGCT plus
AAACAGCGGGATTGTGAGGTAAGC