Table 1. Guide RNA sequences.
Name | Target sequence | Sequence for gRNA cloning | Strand |
---|---|---|---|
DND1_gRNA-1 | GCCCGTTCACCTGCACCAGG NGG | CACCGCCCGTTCACCTGCACCAGG | plus |
AAACCCTGGTGCAGGTGAACGGGC | |||
DND1_gRNA-2 | GACAGGCATCCGCCTGGTGC NGG | CACCGACAGGCATCCGCCTGGTGC | minus |
AAACGCACCAGGCGGATGCCTGTC | |||
DND1_gRNA-3 | GGCTGGGACTACCGTACCTG NGG | CACCGGCTGGGACTACCGTACCTG | plus |
AAACCAGGTACGGTAGTCCCAGCC | |||
DND1_gRNA-4 | GCTTACCTCACAATCCCGCT NGG | CACCGCTTACCTCACAATCCCGCT | plus |
AAACAGCGGGATTGTGAGGTAAGC |