Table 4. Primer sequences for RT-qPCR.
Target | Forward | Reverse | Source |
---|---|---|---|
ACTB | ATAGCAACGTACATGGCTGG | CACCTTCTACAATGAGCTGC | (Irie et al., 2015) |
BLIMP1 | AAACCAAAGCATCACGTTGACA | GGATGGATGGTGAGAGAAGCAA | (Sasaki et al., 2015) |
DND1 | TGCTGGGACAGGGACCTATG | ACGGCCATGGAAGATCACTG | (Irie et al., 2015) |
DNMT3B | TAACTGGAGCCACGACGTAAC | GCATCCGTCATCTTTCAGCCTA | (Sasaki et al., 2015) |
EOMES | AAGGGGAGAGTTTCATCATCCC | GGCGCAAGAAGAGGATGAAATAG | (Yokobayashi et al., 2017) |
GAPDH | CGCTTCGCTCTCTGCTCCTCCTGT | GGTGACCAGGCGCCCAATACGA | (Irie et al., 2015) |
NANOG | AGAGGTCTCGTATTTGCTGCAT | AAACACTCGGTGAAATCAGGGT | (Sasaki et al., 2015) |
NANOS3 | TGGCAAGGGAAGAGCTGAAATC | TTATTGAGGGCTGACTGGATGC | (Sasaki et al., 2015) |
OCT4 | CTGTCTCCGTCACCACTCTG | AAACCCTGGCACAAACTCCA | (Sasaki et al., 2015) |
PRDM14 | TATCATACTGTGCACTTGGCAGAA | AGCAACTGGGACTACAGGTTTGT | (Sasaki et al., 2015) |
SOX17 | TTCGTGTGCAAGCCTGAGAT | TAATATACCGCGGAGCTGGC | (Sasaki et al., 2015) |
SOX2 | TGAATCAGTCTGCCGAGAATCC | TCTCAAACTGTGCATAATGGAGT | (Sasaki et al., 2015) |
STELLA | AAGCCCAAAGTCAGTGAGATGA | GCTATAGCCCAACTACCTAATGC | (Sasaki et al., 2015) |
T | AGCCAAAGACAATCAGCAGAAA | CACAAAAGGAGGGGCTTCACTA | (Sasaki et al., 2015) |
TFAP2C | ATTAAGAGGATGCTGGGCTCTG | CACTGTACTGCACACTCACCTT | (Sasaki et al., 2015) |