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. 2021 Oct 15;16(10):e0258427. doi: 10.1371/journal.pone.0258427

Table 4. Primer sequences for RT-qPCR.

Target Forward Reverse Source
ACTB ATAGCAACGTACATGGCTGG CACCTTCTACAATGAGCTGC (Irie et al., 2015)
BLIMP1 AAACCAAAGCATCACGTTGACA GGATGGATGGTGAGAGAAGCAA (Sasaki et al., 2015)
DND1 TGCTGGGACAGGGACCTATG ACGGCCATGGAAGATCACTG (Irie et al., 2015)
DNMT3B TAACTGGAGCCACGACGTAAC GCATCCGTCATCTTTCAGCCTA (Sasaki et al., 2015)
EOMES AAGGGGAGAGTTTCATCATCCC GGCGCAAGAAGAGGATGAAATAG (Yokobayashi et al., 2017)
GAPDH CGCTTCGCTCTCTGCTCCTCCTGT GGTGACCAGGCGCCCAATACGA (Irie et al., 2015)
NANOG AGAGGTCTCGTATTTGCTGCAT AAACACTCGGTGAAATCAGGGT (Sasaki et al., 2015)
NANOS3 TGGCAAGGGAAGAGCTGAAATC TTATTGAGGGCTGACTGGATGC (Sasaki et al., 2015)
OCT4 CTGTCTCCGTCACCACTCTG AAACCCTGGCACAAACTCCA (Sasaki et al., 2015)
PRDM14 TATCATACTGTGCACTTGGCAGAA AGCAACTGGGACTACAGGTTTGT (Sasaki et al., 2015)
SOX17 TTCGTGTGCAAGCCTGAGAT TAATATACCGCGGAGCTGGC (Sasaki et al., 2015)
SOX2 TGAATCAGTCTGCCGAGAATCC TCTCAAACTGTGCATAATGGAGT (Sasaki et al., 2015)
STELLA AAGCCCAAAGTCAGTGAGATGA GCTATAGCCCAACTACCTAATGC (Sasaki et al., 2015)
T AGCCAAAGACAATCAGCAGAAA CACAAAAGGAGGGGCTTCACTA (Sasaki et al., 2015)
TFAP2C ATTAAGAGGATGCTGGGCTCTG CACTGTACTGCACACTCACCTT (Sasaki et al., 2015)