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. 2000 Feb;20(3):797–804. doi: 10.1128/mcb.20.3.797-804.2000

FIG. 2.

FIG. 2

Expression of the Gq class α subunits Gα15, Gαq, and Gα11 in wild-type and Gα15 KO mice. (A) Expression of Gα15 in mouse hematopoietic cells. The Gα15 C-terminal antibody B861-7 was used to detect Gα15 in membranes obtained from murine resident peritoneal exudate leukocytes (PEL), peritoneal exudate macrophages collected and purified by adherence 5 days after thioglycolate injection (PEM), and total bone marrow (BM) or mouse bone marrow separated into fractions by synchrony elutriation. Fraction 1 (Fr.1) is enriched in lymphocytes and may contain erythrocytes; fraction 2 (Fr.2) contains lymphocytes and blasts; fraction 3 (Fr.3) is enriched in blasts and mature neutrophils, fraction 4 (Fr.4) contains mostly mature neutrophils. rGα15, recombinant Gα15. (B) Expression of Gα15, Gαq, and Gα11 in membranes from hematopoietic tissues of Gα15 wild-type and Gα15−/− mice, using antibodies B861-7, W082, and B825. The liver was used as a negative control for Gα15 and as a positive control for Gαq and Gα11. +/+, Gα15 wild-type mice; −/−, Gα15 KO mice; rGα15, membranes from Gα15 expressed in Sf9 cells; rGα, recombinant Gα15, Gαq, or Gα11 on their respective blots. Five micrograms of membrane protein was loaded per lane except in day 5 peritoneal exudate macrophages (3 μg).