FIG. 2.

Electrophoretic analysis of SDS mitochondrial lysates from the [³⁵S]methionine 30-min-pulse-labeled 3 parental lines, 11 ρ° LL/2-m21 transformants, and 4 ethidium bromide-derived 3A20 subclones (a), kinetics of the ND5 labeling in LL/2 and 3A33 cells (b), and electrophoretic patterns of immunoprecipitates obtained from Triton X-100 mitochondrial lysates of pulse-chased LL/2 and 3A20-4 cells with gamma globulins from an antiserum against the human ND4L subunit (ND4L) or from normal rabbit serum (NS) (c). For details, see Materials and Methods. In panel a, the patterns for the transformants and 3A20-4 are arranged, with minor deviations, in the order of decreasing malate-glutamate-dependent respiration rate.