TABLE 1.
Cell line | Avg activity (nmol/min/mg, ± 2 SEM)b
|
NADH-Q1/NADH-K3Fe(CN)6 (103)c | |
---|---|---|---|
NADH-Q1 oxidoreductase | NADH-K3Fe(CN)6 oxidoreductase | ||
LL/2 | 11.0 (±1.2) | 2,079 (±245) | 5.3 |
3A20-4 | <0.15 | 1,693 (±150) | <0.089 (<1.7) |
The values for NADH-Q1 represents the total activity, with >98% being rotenone sensitive.
Determinations were made in triplicate.
Value in parentheses represents the percentage of normalized NADH-Q1 oxidoreductase activity in 3A20-4 relative to that in LL/2.