Skip to main content
. 2021 Nov 11;184(23):5699–5714.e11. doi: 10.1016/j.cell.2021.10.011

Figure 5.

Figure 5

Short dosing interval with the BNT162b2 (Pfizer-BioNTech) vaccine elicits distinct NAb titer profiles against SARS-CoV-2 variants of concern that decline over time, whereas T cell responses are maintained

(A) NAb titers, measured by FRNT, against the early pandemic Victoria isolate, B.1.351 (beta), P.1 (gamma), and B.1.617.2 (delta) from naive participants in weeks 1 (n = 25), 4 (n = 19), and 13 (n = 20) after the second vaccine in the short interval dose cohort (median dose interval, 3.3 weeks; range, 2.4–4.3). The x axis indicates weeks since dose. Geometric mean neutralizing titers with 95% confidence intervals are shown. Neutralization titers from dose 2 + 1 week (Victoria, B.1.351, P.1, and B.1.617.2) and dose 1 + 4 weeks and 10 weeks (Victoria and B.1.617.2) have been reported previously (Dejnirattisai et al., 2021; Liu et al., 2021; Supasa et al., 2021; Zhou et al., 2021).

(B) Comparison of IFNγ ELISpot responses to S Victoria from cryopreserved PBMCs in 43 naive individuals and 26 pre inf individuals who received the short interval dose 1, 4, and 13 weeks after the second dose.

(C) Comparison of IFNγ ELISpot responses from cryopreserved peripheral blood mononucelar cells (PBMCs) in 40 naive individuals and 42 pre inf individuals matched for responses to S Victoria, spike B.1.35/beta, and S P.1/gamma. Individuals received the long interval dosing regimen, and samples were taken 4 weeks after the second dose. Data are shown as spot forming units (SFU) per million PBMCs.

Time points for (A) were compared with Kruskal-Wallis non-parametric test and Dunn’s multiple comparisons tests. The p values are illustrated above linking lines and fold changes in brackets. Bars represent the median with interquartile range for (B) and (C). Time points were compared with two-tailed Mann-Whitney tests for (A) and (B), and paired comparisons were performed using Wilcoxon matched pairs signed rank test for (C), with fold change values referring to the p value comparisons directly below. Gray circles, naive individuals; red circles, pre inf individuals.