Figure S4.

Representative gating strategy for ICS analysis, related to Figure 4

(A) Description of gates left-to-right showing sequential gates used in the initial analysis of each subject. Cell doublets were removed using forward scatter (FSC) parameters. Lymphocytes were selected by FSC and side scatter (SSC), CD3 positive but non-Live-dead stained living populations were carried forward to isolate CD4+ and CD8+ T Cells. (B) The gates used to define differences between different cytokine expression and (C) total individual cytokine expression in CD4+ and (D,E) CD8+ respectively. DMSO and PMAI represent negative and positive control conditions to demonstrate the contrast in cytokine expression. These were also used in setting the gate boundaries in each subject for the analysis of SARSCoV2 Spike protein responses.