| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Purified NA/LE mouse anti-human CD28 (Clone: CD28.2) | BD Biosciences, UK | Cat#555725; RRID:AB_396068 |
| Purified NA/LE mouse anti-human CD49d (Clone: 9F10) | BD Biosciences, UK | Cat#555501; RRID:AB_2130052 |
| BV510 mouse anti-human CD3 (Clone: UCHT1) | BD Biosciences, UK | Cat#563109: RRID:AB_2732053 |
| Brilliant Violet 421 mouse anti-human CD8 (Clone: RPA-T8) | BD Biosciences, UK | Cat#562428; RRID:AB_11154035 |
| PE mouse anti-human TNFα (Clone: MAb11) | Thermo Fisher Scientific, | Cat#12-7349-82; RRID:AB_466208 |
| PERCP/ Cyanine 5.5 mouse anti-human CD4 (Clone: RPA-T4) | Biologend, UK | Cat#300530: RRID:AB_394493 |
| APC-Cy7 mouse anti-human CD14 (Clone: MφP9) | BD Biosciences, UK | Cat#557831: RRID:AB_396889 |
| FITC mouse anti-human IFNγ Clone: 45-15) | Miltenyi Biotec Ltd | Cat#130-113-492; RRID:AB_2733589 |
| APC mouse anti-human IL-2 (5344.111) | BD Biosciences, UK | Cat#341116; RRID:AB_400574 |
| PE-Cy7 mouse anti-human CD107α (Clone: H4A3) | BD Biosciences, UK | Cat#561348; RRID:AB_10644018 |
| Human FluoroSPOT anti-IgG capture mAb | Mabtech | Cat#FSX-05R-10 |
| Human anti-NP (mAb206) | Dejnirattisai et al., 2021 | N/A |
| Anti-Human IgG (Fc specific)-Peroxidase | Sigma | Cat#A0170; RRID:AB_257868 |
| Bacterial and virus strains | ||
| SARS-CoV-2/P.1 | Dejnirattisai et al., 2021 | N/A |
| SARS-CoV-2/B.1.617.2 | Wendy Barclay and Thushan De Silva | N/A |
| SARS-CoV-2 (Australia/VIC01/2020) | Caly et al., 2020 | N/A |
| SARS-CoV-2/B.1.351 | Public Health England | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| TrueBlue Peroxidase Substrate | Insight Biotechnology | Cat#5510-0030 |
| Dulbecco’s Modified Eagle Medium, high glucose | Sigma-Aldrich | Cat#D5796 |
| Custom synthesized peptides (18-mers) | Mimotopes | http://www.mimotopes.com |
| Dimethyl Sulfoxide | Sigma | Cat#D2650-100ML |
| RPMI-1640 Medium with Sodium bicarbonate, no L-Glutamine | Sigma | Cat#R0883 |
| L-Glutamine | Sigma | Cat#G7513 |
| Penicillin/Streptomycin | Sigma | Cat#P0781 |
| Fetal Bovine Serum | Sigma | Cat#F9665-500ML |
| Lymphoprep | StemCell Technology | Cat#07861 |
| L-Glutamine–Penicillin–Streptomycin solution | Sigma-Aldrich | Cat#G1146 |
| GlutaMAX Supplement | GIBCO | Cat#35050061 |
| Phosphate buffered saline tablets | Fisher Scientific | Cat#12821680 |
| Fetal Bovine Serum | GIBCO | Cat#12676029 |
| Carbonate/bicarbonate capsules | Sigma Aldrich | Cat#C3041-100CAP |
| ProMix CEF peptide pool | Proimmune, Oxford | Cat#PX-CEF |
| Phytohemagglutinin-L | Sigma Aldrich | Cat#11249738001 |
| Carboxymethyl cellulose | Sigma | Cat#C4888 |
| Tween 20 | Sigma Aldrich | Cat#P2287-500ml |
| 1-Step NBT/BCIP Substrate Solution | Life Technologies | Cat#34042 |
| LIVE/DEAD fixable near-IR dead cell stain kit | Thermo Fisher Scientific | Cat#L34975 |
| Perm/Wash Buffer (10x) | BD Biosciences | Cat#554723 |
| Brefeldin A | Merck, UK | Cat#B6542 |
| PMA | Merck, UK | Cat#P1585 |
| Ionomycin calcium salt from Streptomyces conglobatus | Merck, UK | Cat#I0634 |
| DPBS, no calcium, no magnesium | Thermo Fisher Scientific | Cat#14190144 |
| 37% Formaldehyde solution | Merck, UK | Cat#F8775 |
| GIBCO Fetal Bovine Serum, qualified, heat inactivated | Thermo Fisher Scientific, UK | Cat#10500064 |
| RPMI-1640 Medium with sodium biicarbonate but without L-Glutamine | Merck, UK | Cat#R0883 |
| Bovine Serum Albumin (BSA) | Merck, UK | Cat#A9418 |
| Critical commercial assays | ||
| V-PLEX COVID-19 Coronavirus Panel 3 (IgG) Kit | Meso Scale Discovery, Rockville, MD USA | Cat#K15399U-2 |
| V-PLEX SARS-CoV-2 Panel 7 (ACE2) Kit | Meso Scale Discovery, Rockville, MD, USA | Cat#K15440u |
| Human IgA/IgG FluoroSpotFLEX kit | Mabtech | Cat#X-06G05R-10 |
| Human memory B cell stimpack | Mabtech | Cat#3660-1 |
| Human IFNγ ELISpot Basic kit | Mabtech | Cat#3420-2A |
| Deposited data | ||
| Immunogenicity of standard and extended dosing intervals of BNT162b2 mRNA vaccine. Payne et al. | Mendeley Data | https://doi.org/10.17632/fyp26zjgmj.1 |
| Experimental models: Cell lines | ||
| Vero cells | ATCC | Cat#CCL-81 |
| Software and algorithms | ||
| Discovery Bench 4.0 | Meso Scale Discovery, Rockville, MD, USA | Immunoassay Analysis Software | Meso Scale Discovery |
| Prism 8.0 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| IBM SPSS Software 26 | IBM | https://www.ibm.com/us-en/?ar=1 |
| AID ELISpot software 8.0 | Autoimmun Diagnostika | http://www.elispot.com/products/software |
| Flojo 10.7.1 | BD Biosciences | https://www.flowjo.com/ |
| R version 4.0.4 (2021-02-15)–“Lost Library Book” | Web-based open source software | https://www.r-project.org |
| R studio version 1.1.463 | Web-based open source software | https://www.rstudio.com |
| Other | ||
| FacsCanto II cytometer | BD Biosciences UK | N/A |
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