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. 2021 Oct 16;37(5):109929. doi: 10.1016/j.celrep.2021.109929

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© 2021 The Author(s)

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

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Characterization of MERS stem stabilized (SS) spike (S) variants

(A) Side view of the trimeric MERS-CoV globular S ectodomain in a prefusion conformation (PDB: 5W9I) with S1 subunits omitted. One protomer of the S2 subunit is shown as a ribbon diagram with the other two protomers shown in a transparent molecular surface. The regions that refold during the pre-to-post-fusion transition are colored cyan with the rest of S2 in tan. Each inset corresponds to the location of beneficial substitutions in the MERS SS.V1 construct.

(B) SDS-PAGE of MERS-CoV S2-2P and individual S variants on mut11 backbone. Molecular weight standards in kDa are indicated at the left.

(C–E) Size-exclusion chromatography (SEC) traces of purified S variants, grouped by type (C, cavity and aromatic; D, disulfide; E, polar and salt bridge). A vertical dotted line indicates the peak retention volume for S2-2P.

(F) SEC traces for combinatorial disulfide-substituted S variants. One or two additional disulfide substitutions are introduced on the MERS SS.V1 backbone.

See Figures S1 and S2.