Figure 5.
Stem-targeted IgG22 binds to helical stalk region of SARS-CoV-2 S
(A) Representative 2D class averages and 3D reconstructions of Fab22 bound to SARS-CoV-2 S.
(B) Side view of the cryo-EM map of Fab22 bound to SARS-CoV-2 S. The map is colored by local resolution. Fab22 binds to the base of the S.
(C) Model of full-length wild-type SARS-CoV-2 S (PDB: 6XR8) docked into EM map of Fab22 bound to SARS-CoV-2 S. The S protein is colored green, and the helical stalk is colored blue. Zoomed view of the helical stalk has side chains shown in blue and N-linked glycans shown in pink.
(D) Binding of Fab22 to three SARS-CoV-2 S variants captured by RBD-directed IgG S309 is assessed by biolayer interferometry (BLI). Binding data are shown as black lines, and the best fit to a 1:1 binding model is shown as red lines.