REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-MERS-CoV S antibody, G4	PMID: 28807998	N/A
Goat anti-mouse IgG-horseradish peroxidase (HRP) conjugate	SigmaAldrich	Cat # G-21040
Anti-MERS-SS antibody, IgG22	This manuscript	N/A
Anti-MERS-SS antibody, IgG72	This manuscript	N/A
Anti-SARS-CoV S antibody, S309	PMID: 32422645	N/A
Bacterial and virus strains
MERS-CoV EMC/2012	Scobey et al., 2013	N/A
DH5α competent cells	ThermoFisher	Cat# 18258012
SARS-CoV-2/human/USA/WA-CDC-WA1/2020	CDC	GenBank: MN985325.1
Mouse-adapted MERS-CoV EMC derivative, maM35c4	PMID: 29277291	N/A
SARS-CoV-2 MA10	PMID: 33031744	N/A
Chemicals, peptides, and recombinant proteins
3,5,3′5’-tetramethylbenzidine (TMB)	KPL	Cat# 5150-0021
MERS-CoV S-2P protein	PMID: 28807998	N/A
SARS-CoV-1 S-2P protein	PMID: 28807998	N/A
SARS-CoV-2 S-2P protein	PMID: 32075877	N/A
SARS-CoV-2 S-HexaPro protein	PMID: 32703906	N/A
DS-Cav1 protein	PMID: 24179220	N/A
T4 foldon protein	PMID: 28807998	N/A
MERS-CoV SS.V1 protein	This manuscript	N/A
MERS-CoV SS.V2 protein	This manuscript	N/A
MERS peptide- DFQDELDEFFKNVST	This manuscript	N/A
SARS peptide- DSFKEELDKYFKNHT SPDVD	This manuscript	N/A
Bovine serum albumin	Sigma-Aldrich	Cat# A8327
Anti-Human IgG Fc Capture (AHC) Biosensors	FortéBio	Cat# 18-5063
SYPRO Orange Protein Gel Stain	ThermoFisher	Cat# S6651
FreeStyle 293 Expression Medium	GIBCO	Cat# 12338002
OPTI-MEM, Reduced Serum Medium	ThermoFisher	Cat# 11058021
Kifunensine	GlycoSyn	Cat# FC-034
25 kDa linear polyethylenimine	Polysciences	Cat# 3966-2
Critical commercial assays
Luciferase Assay System	Promega	Cat# E1500
Luciferase Cell Culture Lysis 5X Reagent	Promega	Cat# E1531
FuGENE® HD Transfection Reagent	Promega	Cat# E2311
Deposited data
Crystal structure of Fab22 + MERS-CoV S peptide	This manuscript	PDB ID: 7S3M
Crystal structure of Fab22 + SARS-CoV-2 S peptide	This manuscript	PDB ID: 7S3N
EM map of Fab22 + MERS-CoV S	This manuscript	EMDB ID: 25072
EM map of Fab22 + SARS-CoV-2 S	This manuscript	EMDB ID: 25073
Experimental models: Cell lines
Huh7.5 cells	Provided by Dr. Deborah R. Taylor of the US FDA	N/A
Freestyle 293F cells	ThermoFisher Scientific	Cat# R7007
Vero E6 cells	PMID: 14569023	N/A
Vero 81 cells	ATCC	Cat# CCL-81
Experimental models: Organisms/strains
Mus musculus, strain BALB/cJ	Jackson Laboratories	JAX stock #000651
Mus musculus, strain BALB/cAnNHsD (BALB/c)	Envigo	Stock # 047
Mus musculus, strain C57BL/6J	Jackson Laboratories	JAX stock #000664
Mus musculus, strain 288/330+/+	PMID: 31883094	N/A
Recombinant DNA
pCG1-SARS-2-S	PMID: 32142651	N/A
pCG1-SARS-S	PMID: 24023659	N/A
pαH expression plasmid	McLellan Laboratory	N/A
pαH-MERS-SS.V1	This manuscript	N/A
pαH-MERS-SS.V1	This manuscript	N/A
pαH-SARS-CoV-1 S-2P	PMID: 28807998	N/A
pαH-MERS-CoV S-2P	PMID: 28807998	N/A
pαH-SARS-CoV-2 S-HexaPro	PMID: 32703906	N/A
pVRC8400-VH22	This manuscript	N/A
pVRC8400-VL22	This manuscript	N/A
pVRC8400-VH72	This manuscript	N/A
pVRC8400-VL72	This manuscript	N/A
pHR′ CMV-Luc	Graham Laboratory	N/A
CMV/R-MERS-CoV S	Graham Laboratory	N/A
CMV/R-SARS-CoV-1 S	Graham Laboratory	N/A
Software and algorithms
Protein Repair One-Stop Shop (PROSS) server	PMID: 27425410	https://pross.weizmann.ac.il/step/pross-terms/
Octet Data Analysis software	FortéBio	v11.1
GraphPad Prism	(Motulsky and Brown, 2006)	V9.0.2
Biacore X100 Evaluation Software	GE Healthcare	V2.0.1
iMOSFLM	Battye et al., 2011	https://www.mrc-lmb.cam.ac.uk/harry/imosflm/ver721/downloads.html
Aimless	Evans and Murshudov, 2013	www.ccp4.ac.uk/download/
Phaser	McCoy et al., 2007	www.ccp4.ac.uk/download/
COOT	Emsley and Cowtan, 2004	http://bernhardcl.github.io/coot/
Phenix	Adams et al., 2002; Afonine et al., 2018	https://phenix-online.org/
ChimeraX	Goddard et al., 2018	https://www.rbvi.ucsf.edu/chimerax/
cisTEM	Grant et al., 2018	https://cistem.org/
cryoSPARC	Punjani et al., 2017	v2.15.0
Other
Strep-Tactin Superflow resin	IBA Lifesciences	Cat# 2-1206-010
Pierce™ Protein A Agarose	ThermoFisher Scientific	Cat# 20334
Biacore X100 Sensorchip NTA	GE Healthcare	Cat# BR100407
Superose 6 increase 10/300 GL	GE Healthcare	Cat# 29091596
Sigma Adjuvant System	Sigma-Aldrich	Cat# S6322-1VL
Carbon Film 400 Mesh, Cu	Electron Microscopy Sciences	Cat# CF400-Cu-50
C-Flat Holey Carbon Grid CF-1.2/1.3-4C, 400 mesh Cu	Electron Microscopy Sciences	Cat# CF413-50