Fig. 2.

Androgen responsiveness of CRPC-associated miRNAs. Analysis of KLK3 a, miR-21 b, miR-141 c, miR-3195 d, miR-3687 e and miR-4417 f expression in VCaP cells. VCaP cells were cultured for 3 days in androgen-free (CSS) medium, and then incubated in the presence of 0.1, 1 or 10 nM of synthetic androgen R1881. Total RNA was extracted 24 and 48 h after androgen stimulation. KLK3 and miRNA levels were determined by reverse transcriptase real-time PCR analysis. HPRT1 and RNU6 levels were used for normalization purposes. All experiments were repeated at least three times; p-values were calculated with the unpaired t-test (*p < 0.05; **p < 0.01; ***p < 0.001)