Fig. 3.

Effect of CRPC-associated miRNAs on cell survival. PC3 a–c, f and VCaP d–e cells were transfected with miRNA mimics (10–50 nM) or with anti-miR miRNA inhibitors (50 nM). Total RNA was extracted and the relative expression of miR-3687 was determined by stem-loop RT-qPCR analysis, using RNU6 for normalization a, d. Cell viability of miRNA mimic or anti-miR transfected cells was determined at different time points post-transfection b, c, e. Transfected cells were seeded 2 days post-transfection in 96-well plates. Cell viability was determined from day 1 until day 5 (PC3) or day 11 (VCaP), using CellTiter-Glo Assays. Apoptosis of miRNA mimic and anti-miR transfected PC3 cells was determined 2 days post transfection, using a Caspase-3/7 Assay f. NT non-transfected cells. Error bars indicate the standard deviation of at least 2 independent experiments