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. 2021 May 14;39(10):3789–3797. doi: 10.1007/s00345-021-03723-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Effect of CRPC miRNAs on cell migration and cell invasion. Cell migration a was assessed by wound-healing “scratch” assays. Error bars indicate the standard deviation of at least four independent experiments performed in triplicate. Cell invasion b was determined using Matrigel-coated 8-µm cell invasion chambers. PC3 cells were transfected with miRNA mimics (10–50 nM) or with anti-miR miRNA inhibitors. Inhibition of cell invasion by a specific miRNA was calculated by dividing the percentage of invaded cells versus that of NC2 transfected cells. NT non-transfected cells. Error bars indicate the standard deviation of two independent experiments performed in duplicate; p values were calculated with the unpaired t-test (*p < 0.05; ***p < 0.001)