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. 2021 Oct 15;12:6040. doi: 10.1038/s41467-021-26247-2

Fig. 6. Optogenetic stimulation of dmPFC-DMS projections reduces compulsive grooming in SAPAP3 KO mice.

Fig. 6

Bar and line graphs show data means +/− SEM. Source data are provided as a Source data file. *P < 0.05, **P < 0.01, ***P < 0.001. Black lines on bar graph’s represent individual animals. Lines start and stop at individual animal’s average for that graph’s conditions. a Cartoon of viral injection and implant. b Graphical representation of behavior-dependent stimulation experiment (ChR2-KO mice = 7, eYFP-KO mice = 6). c Duration of grooming epochs during behavior-dependent stimulation (two-way RM ANOVA: interaction P = 0.0252, laser P = 0.0241, virus P > 0.05, subject P = 0.0043; Sidak’s multiple comparisons: ChR2-KO on vs off P = 0.0056). d Laser-evoked change in average duration of grooming epochs (unpaired two-tailed t-test P = 0.0252). e Graphical representation of time-dependent stimulation experiment (ChR2-KO = 15, ChR2-WT = 18, eYFP-KO = 10, eYFP-WT = 11). f Grooming duration across 5-min blocks (two-way RM ANOVA: interaction P = 0.0430, laser P < 0.0001, genotype P = 0.0018, subject P < 0.0001; Sidak’s multiple comparisons: baseline ChR2-WT vs ChR2-KO P = 0.0043, laser off ChR2-WT vs ChR2-KO P = 0.0007). g Laser-evoked reduction in grooming behavior relative to baseline (one-way ANOVA P = 0.0104; Tukey’s multiple comparisons: eYFP-KO vs ChR2-KO P = 0.0120). h Following 2 weeks of repeated stimulation, KO-ChR2 mice have a significant and sustained reduction in grooming behavior 4 days after the last laser stimulation session (two-way RM ANOVA: interaction P = 0.0354, time P = 0.0070, experimental group P < 0.0001, subject P = 0.0190; Sidak’s multiple comparisons: ChR2-KO baseline vs 4 days post-stimulation P = 0.0015). i Four days after the final stimulation, KO-ChR2 mice show a significantly greater decrease from baseline grooming behavior than WT ChR2 mice (unpaired two-tailed t test P = 0.0230).