Table 3.

Long-read (ONT) sequencing summary and haplogroup classification results for the samples. The results of the short-read whole-genome sequencing (WGS) mtDNA classification are also shown.

Samples	ONT sequencing		Variant-calling		Reference-based assembly		De novo assembly		Hybrid de novo assembly		Short-read WGS
	Mapped reads	Depth of coverage	Variants	Haplogroup	Variants	Haplogroup	Variants	Haplogroup	Variants	Haplogroup	Variants	Haplogroup
CAN03	3,065	581	14	H1ao1	69	H1ao1	119	H1ao1	16	H1ao1	14	H1ao1
CAN06	2,981	566	36	J2a2d	141	J2a2d	154	J2a2d	46	J2a2d	44	J2a2d
CAN07	2,343	444	15	H6a1b2	132	H6a1b2	175	H6a1b	17	H6a1b2	14	H6a1b2
CAN09	2,126	358	33	U4c1	66	U4c1	602	U4c1	37	U4c1	37	U4c1
CAN17	4,209	821	10	H2a2	59	H + 16,189	41	H + 16,189	15	H + 16,189	12	H + 16,189
CAN23	1,737	532	29	K1a1b1	69	K1a1b1	78	K1a1b1	37	K1a1b1	35	K1a1b1
CAN31	1,776	398	20	U6b1a1	67	U6b1a1	119	U6b1a1	28	U6b1a1	26	U6b1a1
CAN34	4,256	910	34	L3f1b1a	90	L3f1b1a	174	L3f1b1a	42	L3f1b1a	43	L3f1b1a