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. 2021 Oct 15;11:20531. doi: 10.1038/s41598-021-00085-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cytotoxicity evaluation of the nanostructures. EPG85.257-RDB and HeLa-RDB viability after 72 h incubation with nanoparticle-free media (a), MSN (b), NH₂-MSN(c), MSN-CS(d), MSN-CS-PEG-folate (e), MSN-CS-PEG-TAT (f), and MSN-CS-PEG-folate/TAT (g). Nanoparticles were found to be biocompatible, and the treatment concentration should not exceed 100 μg/ml. Data showed that nanoparticles were biocompatible, and the treatment concentration should not be exceeded 100 μg/ml. *p < 0.05 denote significant differences between the control and treatment samples according to the one-way ANOVA (non-parametric Kruskal–Wallis analyses). CS Chitosan, PEG polyethylene glycol, TAT trans-activator of transcription.