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. 2021 Oct 15;12(10):951. doi: 10.1038/s41419-021-04241-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Western Blot analysis evaluating p27 and phosphorylated Src (pSrc^Y416) expression in CRC cell lines harboring KRAS mutation (SW480, SW620) or not (HT29, Caco-2, Colo320), as indicated. Cells were collected after serum starvation (0) and release with FBS for 15’, as indicated. GAPDH was used as loading control. B Left: Representative images of Src and pSrc^Y416 expression evaluated by IHC in CRC samples harboring or not mutation of KRAS and BRAF, as indicated. Right: Graph reports the percentage of CRC samples showing activation of Src (pSrc^Y416) compared to the mutational status of RAS.