Fig. 4.

The preferential binding of RM-LIP to inflamed endothelial cells in vitro. (A) Representative fluorescence images of different liposomes (red) and HUVECs (blue) in the untreated group and the LPS treated group (scale bar: 50 µm). (B) Representative western blots of VCAM-1 from untreated HUVECs and LPS treated HUVECs, and β-actin as an internal reference protein, and quantitative analysis results were presented by a histogram. (n = 3, ***P< 0.001). Flow cytometry analysis of the binding of RM-LIP to LPS-treated HUVECs (C) and untreated HUVECs (D) (n = 3, **P< 0.005).