Fig. 5.

Targeting effect of RM-LIP to SCI. (A) and (B) Representative ex vivo images of mice 1, 4, 8 and 12 h after injection of DiR-labeled liposomes: normal mice treated with RM-LIP/DiR (i), SCI mice treated with PEG-LIP/DiR (ii), SCI mice treated with RM-LIP/DiR (iii), SCI mice treated with blocked RM-LIP/DiR(iv). The organs were organized in the following order (from left to right): spinal cord, brain, heart, lung, liver, spleen, kidney; (C) Coumarin 6 concentration in various tissues at different time points after intravenous injection of PEG-LIP/C6 and RM-LIP/C6. Data were presented as mean ± SD (n = 3, *P< 0.05, **P< 0.005); (D) Fluorescence images of different liposomes (green) and GFAP (red) in normal spinal cords or injured spinal cords (Scale bar: 100 µm).