Fig. 7.

The influence of different macrophage polarization states. The expression of Mac-1 (A) and integrin α4 (B) in different macrophage subtypes were analyzed by flow cytometry; (C) The number of Mac-1 and integrin α4 molecules on a cell surface of macrophages by using a QuantumTMR-PE MESF kit to standardizing fluorescence intensity (n = 3); (D) Representative fluorescence images of different liposomes (red) and HUVECs (blue) in the LPS-treated HUVECs (scale bar: 50 µm); (E) Quantification of the fluorescence intensity of preparations by using flow cytometry (n = 3); (F) Representative ex vivo images of mice in 1, 4, 8 and 12 h after injection of DiR-labeled liposomes: RM-LIP/DiR (i), RM1-LIP/DiR (ii), RM2-LIP/DiR (iii).