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. Author manuscript; available in PMC: 2021 Oct 16.
Published in final edited form as: Methods Cell Biol. 2020 Feb 14;155:295–319. doi: 10.1016/bs.mcb.2019.11.008

Figure 2. Measurement of superoxide levels by DHE staining in cells with PQ treatment.

Figure 2.

(A) Representative images of DHE staining to detect superoxide in living yeast cells. Mitochondrial superoxides (red) were visualized using DHE in cells incubated +/− paraquat (2.5 mM) for 8 hr. Mitochondria (green) were visualized by tagging a mitochondrial marker protein (CIT1) at its chromosomal locus with GFPEnvy. Scale bar, 2 μm. (B) Quantification of cellular or mitochondrial superoxide levels by measuring mean DHE intensity. Colocalization of DHE-stained superoxides and mitochondria using Pearson’s correlation coefficient. (n>250 cells for each strain, two-tailed t-test,**p<0.01, ****p < 0.0001).