Pros	Cons
Imaging of mitochondrial redox state in living cells.	Oxidized mito-roGFP photobleaches quickly. Therefore, this reagent cannot be used for time lapse imaging.
As a genetically encoded sensor, roGFP can be introduced and maintained in cells without permeabilization.	Mito-roGFP does not offer the temporal resolution to detect bursts of ROS associated with ROS signalling.
mito-roGFP1 offers subcellular resolution and can detect heterogeneity in the redox state of mitochondria within individual cells.
mito-roGFP1 is a ratiometric biosensor, and is not affected by changes in concentration or variability in sample thickness or illumination.