Table 2. Oligonucleotide primers used for the detection of influenza viruses (H5 and H9), NDV, and IBV by RT-PCR assay.

Target gene fragment	Primer	Sequence (5′-3′)	Amplicon size (base pair)	Reference
H9	H9-F	ATCGGCTGTTAATGGAATGTGTT	221	[13]
	H9-R	TGGGCGTCTTGAATAGGGTAA
N2	N2-F	CTCCAATAGACCCGTACTAT	460	[14]
	N2-R	CCTGAAGTCCCACAAAATAC
H5	H5-F	ACAAAGCTCTATCAAAACCCAAC	499	[13]
	H5-R	TACCCATACCAACCATCTACCAT
ND	ND-F	TGGAGCCAAACCGCGCACCTGCGG	766	[15]
	ND-R	GAGGATGTTGGCAGCAT
IB	IB-F	GCTTTT GAG CCT AGC GTT	149	[16]
	IB-R	GCCATGTTGTCACTGTCTATT
HA	HA9-F	CAAGATGGAAGTAGTATCACT	1,683	[10]
	HA9-R	TTGCCAATTATATACAAATGT