Figure 5.

Expression of granzyme B (GZMB) and perforin on a subset of CD69+ anti-SARS-CoV-2 CAR-Ts

(A) Mean fluorescent intensity (MFI) values of GZMB and perforin expression on Flag-28Z, CR3014-28Z, CR3022-28Z, CR3022-8a-28Z, CR3022-CH3-28Z, and CR3022-IgG4-28Z CAR-Ts in response to 293-ACE2-RBD cells after 18 h of co-culture. Data are shown as mean ± SD of triplicate samples. ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 (compared with Flag-28Z by one-way ANOVA, n = 4). (Upper panel) GZMB; (lower panel) perforin.

(B) viSNE plots for CD69, GZMB, and perforin expression in SARS-CoV-2 CAR-Ts following incubation with 293-ACE2 (upper panels) or 293-ACE2-RBD (lower panels) (n = 4).

(C) Histogram showing relative MFI values for GZMB (upper panel) and perforin (lower panel) in viSNE islands i, ii, and iii (n = 4).

(D) Percent representation of CD69^hiGZMB⁺ perforin⁺ subset in response to 293-ACE2 and 293-ACE2-RBD cells (n = 4).