Fig. 4.

miR -140-3p bound to lncRNA snHG12 in GC tissues and reduced its stability. A Binding site of miR-140-3p and SNHG12 predicted using the Starbase database. B–C The binding relationship of 140-3p and SNHG12 in GC cells was verified using dual luciferase assay and RIP. D–E Expression of SNHG12 in gastric adenocarcinoma was predicted using the Starbase and UALCAN databases. F The relation between SNHG12 and the prognosis of GC patients was analyzed using Kaplan–Meier Plotter database. G The expression of SNHG12 in GC tissues and adjacent tissues was detected using RT-qPCR. H, I The expression of SNHG12 in GC cells was detected using RT-qPCR, N = 6. J The expression of SNHG12 in transplanted tumor tissues was detected using RT-qPCR. K Relevance between miR-140-3p and SNHG12 was analyzed by Pearson correlation analysis. L After GC cells with low expression of miR-140-3p were treated with actinomycin D, the half-life period of SNHG12 was detected using RT-qPCR. The cell experiment was repeated 3 times independently. Data in B, C, H, K were presented as mean ± standard deviation. Comparison between two groups in panels G, I, and J was performed using the t-test. Comparison of data in H was analyzed by using one-way ANOVA and comparison of data in B, C, L was analyzed by using two-way ANOVA, followed by Tukey's multiple comparisons test or Sidak's multiple comparisons test. **p < 0.01. LV-oe-miR: The lentiviral overexpression vector of miR-140-3p; LV-oe-NC: negative control of lentiviral overexpression vector; inhibitor: miR-140-3p inhibitor