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. 2021 Oct 16;21:537. doi: 10.1186/s12935-021-02245-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — SNHG12 bound to RNA-binding protein HuR and induced transportation of HuR from the nuclei to the cytoplasm. A, B The location of SNHG12 was analyzed using subcellular fraction assay and RNA FISH; C The binding probability between SBHG12 and HuR was predicted using RNA–Protein Interaction Prediction (RPISeq) database. D The binding between SNHG12 and HuR was analyzed using RIP assay. E, F The protein expression of HuR in GC tissues and cells was detected by western blot. After the three designed SNHG12 shRNA were treated with AGS cells, NC shRNA was used as the control. G The expression in SNHG12 in AGS cells was detected by RT-qPCR. H The protein expression of HuR in GC cells was detected by western blot. I The aggregation of HuR in the cytoplasm was detected by immunofluorescence. The experiment was repeated three times independently. Data were presented as mean ± standard deviation. Comparison between data in panel E was analyzed by using the t-test. Comparison among groups in panels D, F, G, H was analyzed using one-way ANOVA, followed by Tukey's multiple comparisons test. *p < 0.05, **p < 0.01. sh-NC: NC shRNA; sh-SNHG12: SNHG12 shRNA

Fig. 6 — SNHG12 bound to RNA-binding protein HuR and induced transportation of HuR from the nuclei to the cytoplasm. A, B The location of SNHG12 was analyzed using subcellular fraction assay and RNA FISH; C The binding probability between SBHG12 and HuR was predicted using RNA–Protein Interaction Prediction (RPISeq) database. D The binding between SNHG12 and HuR was analyzed using RIP assay. E, F The protein expression of HuR in GC tissues and cells was detected by western blot. After the three designed SNHG12 shRNA were treated with AGS cells, NC shRNA was used as the control. G The expression in SNHG12 in AGS cells was detected by RT-qPCR. H The protein expression of HuR in GC cells was detected by western blot. I The aggregation of HuR in the cytoplasm was detected by immunofluorescence. The experiment was repeated three times independently. Data were presented as mean ± standard deviation. Comparison between data in panel E was analyzed by using the t-test. Comparison among groups in panels D, F, G, H was analyzed using one-way ANOVA, followed by Tukey's multiple comparisons test. *p < 0.05, **p < 0.01. sh-NC: NC shRNA; sh-SNHG12: SNHG12 shRNA