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. 2021 Oct 16;21:537. doi: 10.1186/s12935-021-02245-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Overexpression of FAM83B reduced the inhibition of overexpression of miR-140-3p on proliferation, invasion, and migration of GC cells. FAM83B pcDNA was transfected into AGS cells. A mRNA level of FAM83B was in AGS cells was detected by RT-qPCR. Joint intervention with lentiviral overexpression vector of miR-140-3p was performed. The proliferation of cells was detected using CCK-8 assay (B) and colony formation assay (C). D, E The invasion and migration of cells were detected by Transwell assays. The experiment was repeated 3 times independently. Data were presented as mean ± standard deviation. Comparison between data in panel A was analyzed using the t-test. Comparisons among data in panels C–E were analyzed by using one-way ANOVA and comparison among data panel B was analyzed by using two-way ANOVA followed by Tukey's multiple comparisons test. **p < 0.01. LV-oe-miR: the lentiviral overexpression vector of miR-140-3p; pc-NC: NC pcDNA; pc-FAM83B: FAM83B pcDNA