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(A) Anti-Tub immunoblot of whole cell lysates from CHO cells transfected with the empty vector (Vec; lanes 1 and 2), HA-Tub (WT; lanes 3 and 4), or HA-mutant Tub (Mut; lanes 5 and 6). (B) Brain lysates were resolved by SDS-PAGE and probed with the anti-Tub antiserum as for Fig. 3. Each lane represents an individual mouse of the indicated genotype. KO, knockout; MC4R, melanocortin 4 receptor.
