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Dioscin abolishes the accumulation of ROS production in H9C2 cells. Representative images of double staining of H9C2 cells with dihydroethidium (DHE) (red) and DAPI (blue) (bar = 50 μm), n = 3 each group. (b) The quantification of relative expression of reactive oxygen species. (c) The mRNA expression of Nox2. n = 6 each group. Data are mean ± SD. ∗P < 0.05 vs. control group, #P < 0.05 vs. H2O2+reoxygen group.
