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TUTase activity sediments with RNP complexes. Mitochondrial extract was sedimented on a 10 to 30% glycerol gradient and fractionated into 16 fractions, and TUTase activity was measured by monitoring the incorporation of [α32P]UTP into yeast total tRNA by using the trichloroacetic acid assay as described in Materials and Methods. Gradient fractions containing complexes I (19S) and II (35S to 40S) are indicated.
