FIG. 1.

Lmx1.1 and E47/Pan1 give greater transcriptional synergy than PDX-1 and E47/Pan1. BHK-21 cells were transfected with a reporter plasmid (pFOXluc.prl.5FF1) containing five copies of the insulin E2A3/4 minienhancer linked to the minimal prolactin promoter driving luciferase gene expression and with expression plasmids containing the CMV promoter driving expression of the cDNAs shown. Luciferase activity in the cells transfected with the reporter plasmid and the parent expression plasmid without insert is set to 1.0.