FIG. 10.

HMG I(Y) contributes to the synergy of PDX-1 and E47/Pan1. (A) Transfections were performed in BHK-21 cells as for Fig. 1A, except for the addition of increasing amounts (0.2 and 0.4 μg) of the HMG I(Y) expression plasmid. Luciferase activity in the cells transfected with the reporter plasmid and the pBAT12 plasmid with no insert is set to 1.0. (B) High Five insect cells were transfected with the pFOXluc.prl.5FF1 reporter construct (1.0 μg) as for panel or with reporter constructs with mutations of the E2 (pFOXluc.prl.5mC1) or A3/4 (pFOXluc.prl.5mEF1) sites. Expression plasmids containing the CMV promoter driving expression of the cDNAs for PDX-1 (100 ng), E47/Pan1 (100 ng), or HMG I(Y) (20, 100, or 500 ng) were cotransfected. Luciferase activity in the cells transfected with the reporter plasmid and the parent expression plasmid without insert is set to 1.0.