FIG. 2.

Excess PDX-1 decreases synergy with E47/Pan1, and excess E47/Pan1 decreases synergy with Lmx1.1. (A) The pFOXluc.prl.5FF1 reporter plasmid was cotransfected with 0.02 μg of the E47/Pan1 expression plasmids and increasing amounts (0.01, 0.02, 0.1, 0.2, and 0.5 μg) of the PDX-1 or Lmx1.1 expression plasmid into BHK-21 cells. Luciferase activity in the cells transfected with the reporter plasmid and the E47/Pan1 expression plasmid alone is set at 1.0. (B) The reporter plasmid was cotransfected with 0.2 μg of the PDX-1 or Lmx1.1 expression plasmid and increasing amounts (0.05, 0.1, 0.2, 0.6, and 1.0 μg) of the E47/Pan1 expression plasmid into BHK-21 cells. The luciferase activity in the cells transfected with the reporter and the PDX-1 or Lmx1.1 expression plasmid alone is set at 1.0.