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. 2021 Sep 14;20(10):e13476. doi: 10.1111/acel.13476

FIGURE 3.

FIGURE 3

Effects of S3QELs in intestinal‐specific knockdown of superoxide dismutases (Sod1 and Sod2) in Drosophila reared on 5.0% YE diet. Intestinal enterocyte‐specific knockdown of cytosolic Sod1 or mitochondrial Sod2 was initiated 5 days after eclosion when flies at 18℃ were transferred to 5% YE with either 8 μM S3QEL or DMSO vehicle at 29℃. Effect of S3QELs on intestinal permeability (a, f), number of intestinal apoptotic cells (b, g), and lifespan (c, h), and relationships between median lifespan and intestinal permeability and intestinal apoptotic cell number (d, e, i, j) under Sod1 (a, b, c, d, e) and Sod2 (f, g, h, i, j) intestinal enterocyte knockdown. Data are means ± SEM of n = 3 biological replicates each using 200 flies (a, c, f, h), or 12 intestines (b, g). *p < 0.05, **p < 0.005, ***p < 0.0001 by three‐way ANOVA (a, f), or one‐way ANOVA with Dunnett's multiple comparisons test (b, g). Lifespan curves in (c, h) were analyzed using the Cox proportional hazards model. Shaded boxes in lifespan graphs (c‐h) indicate the 5‐day post‐eclosion period before flies were transferred to 5% YE. Lines in (d, e, i, j) were fit using linear regression. F, F‐value; p, p‐value; coef, coefficient