FIGURE 1.

SIRT1 is downregulated in AMA term placenta and is associated with placental senescence. (a) Western blot analysis of the expression of SIRT1–7 in human term placental tissues (n = 6 in the control group and AMA group). (b) IF staining of SIRT1 (green) and CK7 (red) in frozen sections of human term placentas; nuclei were counterstained with DAPI (blue). Scale bar: 100 μm. (c) Western blot analysis of the levels of acetyl‐P53, total P53, and P21 in human term placentas (n = 6 in the control group and AMA group). (d) SAβG staining of human term placental sections. Scale bar: 200 μm. AU, arbitrary unit. Data were analyzed using Student's t‐test, *p < 0.05. All data are presented as the mean ± SEM. All experiments were performed in triplicate