FIGURE 3.

Advanced maternal age is associated with adverse birth outcomes and downregulation of SIRT1 in the mouse placenta. (a) The mating plan for the animals. (b) Average litter size. (c) Birth weight of viable fetuses. (d) Placental weight of viable fetuses among different mating groups. (e) H&E staining of sections from E13.5 placentas from different mating groups. Jz, junctional zone; Lz, labyrinth zone. Scale bar: 400 μm. Western blot analysis of the levels of SIRT1, acetyl‐P53, P53, and P21 in (f) E13.5 and (g) E18.5 mouse placentas from different mating groups (n = 3 biologically independent animals from each group). (h) SAβG staining of E13.5 mouse placental sections from different mating groups. Jz, junctional zone; Lz, labyrinth zone. Scale bar: 400 μm. AU, arbitrary unit. Data were analyzed using one‐way ANOVA, followed by Tukey's or Sidak's multiple comparison tests, n = 6; *p < 0.05; **p < 0.01; ***p < 0.001 vs. Young♂ x Young♀ group; #p < 0.05; ##p < 0.01; ###p < 0.001 vs. Aged♂ x Young♀ group. All data are presented as the mean ± SEM. All experiments were performed in triplicate