FIGURE 4.

SIRT1 deficiency induces senescence and impairs the migration and invasion of trophoblast cell lines. (a) Western blot analysis of SIRT1 expression in HTR8/SVneo cells. (b) Western blot analysis of acetyl‐P53, P53, and P21 in HTR8/SVneo cells. (c) SAβG staining of HTR8/SVneo cells. Scale bar: 200 μm. (d) Wound‐healing assay; scale bar: 100 μm. (e) Transwell assay of HTR8/SVneo cells treated with SRT1720 or nicotinamide (NAM) and transfected with shSIRT1. (f) Migration (dotted line circled area) of EVTs induced from human first‐trimester villous explants treated with shSIRT1, SRT1720 and NAM. Scale bar: 200 μm. Data were analyzed using one‐way ANOVA; *p < 0.05, **p < 0.01, ***p < 0.001. All data are presented as the mean ± SEM. All experiments were performed in triplicate