Fig. 1.

Downregulation of AP1 and AP2 subunits in AAGAB KO cells. (A) Diagrams of AP1 and AP2 adaptors. Despite their similarity in overall structure and configuration, subunits of adaptor proteins such as AP1, AP2 and AP3 are not functionally interchangeable, except for the β subunits of AP1 and AP2 (Traub and Bonifacino, 2013). (B) Representative immunoblots showing expression of the indicated proteins in WT and AAGAB KO HeLa cells. M.W., molecular weight. Blots shown are representative of three experiments. (C) Relative expression levels of AP1, AP2, AP3, COPI and COPII in AAGAB KO HeLa cells measured by mass spectrometry. Data are presented as percentage of total expression levels in WT cells. Average values of two biological replicates are shown. Error bars indicate s.d. The full dataset of the whole-cell proteomic analysis is shown in Table S1.