FIG. 7.
Individual mutations in the HCFVIC domain have different effects on association with VP16 and LZIP. A cell-based recruitment assay was performed with transiently transfected 293T cells. A Gal4-responsive luciferase reporter gene (p5xGal-E1B-luc) was transfected into 293T cells by electroporation together with 1 μg of wild-type or mutant version of pCGNGal(1-94)HCF-1N380 and 500 ng of pCGTVP16ΔC or pCGTLZIPN154, as indicated. The values are the average of three independent transfections, and the standard deviation from the mean is indicated by error bars. Fold activation was calculated relative to the activity of wild-type pCGNGal(1-94)HCF-1N380 cotransfected with pUC119.